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Subject: Looking for techniques to perform surface sterilization and determining surface area
From: Dennis Bonal <[log in to unmask]>
Reply-To:Dennis Bonal <[log in to unmask]>
Date:Wed, 13 Feb 2008 20:59:40 +0000

text/plain (21 lines)

My name is Dennis Bonal and I am currently a graduate student at C.W. Post 
of Long Island University.  
I am in the process of doing an experiment with various types of macroalgae.  
I am using a few filamentous species and I am looking to see if there are any 
suggestions to performing surface sterilization.  Such as if I should use dilute 
bleach.  I know this method has been done with larger algal species but I was 
not sure about filamentous species.
The other question is I am currently using a dye method to determine the 
surface area of my algal species. This method, described by Hanna Stewart 
and Robert Carpenter in 2003, did not use very fine, filamentous species.  I am 
looking for another way to determine surface area or if anyone knows a 
method that is accurate with looking at filamentous species.  
The species I am currently looking at are Dasya baillouviana, Spyridia 
filamentosa, and various species of Polysiphonia.  Any information would be 
gladly appreciated.

Dennis Bonal
[log in to unmask]

Stewart, H. L., & Carpenter R. C. 2003. The effects of morphology and water 
flow on photosynthesis of marine macroalgae. Ecology. 84(11): 2999-3012. 

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