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Subject: Bigelow Laboratory 2005 course series, "Foundations of Marine Science"
From: "William M. Balch" <[log in to unmask]>
Reply-To:William M. Balch
Date:Wed, 12 Jan 2005 10:38:12 -0500

text/plain (164 lines)

January 2005

Dear Colleagues,

      Bigelow Laboratory is pleased to announce its course offerings for 
2005.   Five courses at the upper undergraduate level/graduate level are 
planned.  Courses carry 2 or 3 graduate credits from the University of New 
England.   Applications for all courses are now being accepted; please be 
sure to note the application deadline for each course.   Course 
participation for the courses will be limited to 8 - 12 individuals in 
order to provide maximum exposure for the student.  Participants will be 
chosen based upon application materials and the date the deposit is 
received. To register for the course or for further information, please 
contact Ms Jane Gardner at the email address below. Also see the Bigelow 
Laboratory for Ocean Sciences web site at .

Please forward this message to any interested individuals.

Thank you very much.


William Balch

Senior Research Scientist
Course Coordinator
Bigelow Laboratory for Ocean Sciences

P.S. Please excuse any duplicate mailings.


Course Offerings

  Phytoplankton Culture Techniques

  18-24 May, 2005; 2 credit hours; application deadline 1 March 2005

  Dr. Robert Andersen and Dr. Michael Sieracki
Bigelow Laboratory for Ocean Sciences

  The Provasoli-Guillard National Center for Culture of Marine 
Phytoplankton will offer an intensive seven-day course covering basic and 
advanced techniques for isolating, growing and cryopreserving marine 
phytoplankton.  The course is designed for academic graduate students and 
faculty members as well as aquaculturists.  Isolation methods include 
direct single-cell isolation by micropipette, agar plating and flow 
cytometry and indirect methods such as dilution techniques and 
enrichments.  Preparation and sterilization of various culture media will 
be described. Instructors will provide methods for culture purification, 
including physical (e.g. single-cell "washing"), chemical (e.g. antibiotic 
treatments) and automated (e.g., flow cytometry) approaches.  Students, in 
groups no larger than 5-6, will participate in flow cytometric laboratory 
exercises for isolating and purifying culture strains and in 
cryopreservation laboratory exercises.  Cryopreservation will include 
discussion of cryoprotectants, methods for freezing (simple techniques to 
computer-assisted control-rate freezers) and methods for thawing frozen 
cells.  Other topics will include factors affecting culture health, such as 
light, temperature, salinity, nutrients and aeration.   Mass culturing will 
also be addressed, and numerous species from the CCMP collection will be 
available for study.  A copy of the book Algal Culturing Techniques will be 
supplied to each student.

Microzooplankton Ecology
6-10 June, 2005; 2 credit hours; application deadline 1 March 2005

Dr. Diane Stoecker, Horn Point Laboratory
University of Maryland Center for Environmental Science

This one-week lecture course will provide the fundamentals of 
microzooplankton ecology as well as the latest techniques for their 
study.  Topics to be covered include: functional ecology of heterotrophic 
flagellates, planktonic ciliates, heterotrophic-mixotrophic 
dinoflagellates, techniques for microzooplankton culturing , observation 
and enumeration, microzooplankton behavior and life history 
strategies,  techniques for estimation of growth and grazing rates, 
mixotrophy and plastid retention, food web dynamics, impact on C, S and N 
cycles, and challenges of incorporating microzooplankton into 
models.     Practical examples will be provided and integrated with the 
students' specific interests.

Early Life History of Marine Fishes
1-17 August, 2005; 3 credit hours; Application deadline 1 May 2005

John E. Olney, College of William and Mary
Edward D. Houde, University of Maryland Center for Environmental Science
*Course to be held at the Marine Science Center of the University of New 
England, Biddeford, ME

This graduate level lecture and laboratory course offers a comprehensive 
view of the biology and taxonomy of early life stages of fishes.  Pelagic 
eggs, larvae, and newly-transformed juveniles are abundant and diverse 
components of aquatic ecosystems that are vulnerable to biotic and abiotic 
stress.  Morphological variation throughout ontogeny reflects ecological 
roles and systematic relationships.  This class is intended for students 
with an interest in fish ecology, fisheries science, ichthyology, or 
biological oceanography, and an appropriate background in those disciplines 
is required.  For further information, visit  Note, to register for this 
specific course only, contact Mike Dunnington at the University of New 
England ([log in to unmask], or 207-283-0170, ext. 2671).

Small-Scale Physical-Biological Interactions in the Plankton

  15-19 August 2005;  2 credit hours; application deadline 15 May 2005

George Jackson, Texas A&M University
Thomas Kiorboe,  Danish Institute for Fisheries Research
David Fields, Bigelow Laboratory for Ocean Sciences

The adaptations of planktonic organisms, from viruses and bacteria to larval
fish, can be understood only in the context of the immediate physical and
chemical environment in which they live. Nutrient uptake, motility patterns,
feeding and encounter rates, signal transmission and perception are all
constrained by often non-intuitive interactions between organism biology and
small-scale physical and chemical characteristics of the fluid environment
(e.g. viscosity, fluid motion, diffusion). This course will examine the life
and interactions of planktonic organisms in their small-scale world through
lectures and laboratory and computer exercises and demonstrations.

Aquatic Cytometry: Applications of Flow and Imaging Cytometry to the 
Aquatic Sciences

  Date 12 - 16 September 2005; 2 credit hours; application deadline 15 June 

Dr. Michael  Sieracki and Dr. Nicole Poulton
Bigelow Laboratory for Ocean Sciences

This course will cover the application of flow and imaging cytometry 
techniques for the study of marine particles with the emphasis on 
planktonic microorganisms - including prokaryotes, protozoa, and 
phytoplankton.  Emphasis will be on the basics of flow cytometry and 
sorting, image analysis with fluorescence microscopy, and imaging-in-flow 
technology.  There are hands-on operation opportunities with our BD 
FACScan, DAKO-Cytomation Mo Flo sorter, and FlowCAM.  Participants are 
encouraged to develop small projects.  The resources of the Center for 
Culture of Marine Phytoplankton ( and the flow and 
imaging cytometry instruments here at Bigelow are available for the course 
and individual projects.  See and for more information.

For more information on any of these courses, please contact Ms Jane 
Gardner, Bigelow Laboratory for Ocean Sciences, POB 475, W. Boothbay 
Harbor, ME.  04575; Tel. 207-633-9600; email-   [log in to unmask]


William M. Balch
Senior Research Scientist
Bigelow Laboratory for Ocean Sciences
W. Boothbay Harbor, ME  04575   U.S.A.

tel (207) 633-9600                              e-mail: [log in to unmask]
fax (207) 633-9641                              Web: 

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